Optimization of Aspergillus niger α-amylase Activity for Enhanced Glucose Production from Cassava Starch

The aim of this study was to optimize the hydrolytic activity of A. niger α-amylase on cassava starch. Isolation of Aspergillus niger, determination of α-amylase activity, α-amylase production and extraction were performed using standard protocols. Parameters such as pH, temperature, substrate concentration were studied using unifactorial approach. pH was varied from 3.6-5.6, temperature 30-80ºC, substrate concentration 0.3-1.5 g/l. In conclusion, for optimal utilization of α-amylase in the production of numerous products of economic significance, the outcome of this work can be relied upon to boost production of glucose and accessory products from starch.