Ravanbakhsh, Asma and Mahdavi, Majid and Jalilzade-Amin, Ghader and Javadi, Shahram and Maham, Masoud and Mohammadnejad, Daryosh and Rashidi, Mohammad Reza (2016) Acute and Subchronic Toxicity Study of the Median Septum of Juglans regia in Wistar Rats. Advanced Pharmaceutical Bulletin, 6 (4). pp. 541-549. ISSN 2228-5881
![[thumbnail of APB-6-541.pdf]](http://research.manuscritpub.com/style/images/fileicons/text.png)
APB-6-541.pdf - Published Version
Download (824kB)
Abstract
Purpose: Median septum of Juglans regia L. (Juglandaceae) with anti-diabetic effects has been used in Iranian traditional medicine. The present study estimates both oral acute and subchronic toxicities. Methods: In the oral acute toxicity study, female Wistar rats were treated with doses of 10, 100, 1000, 1600, 2900 and 5000 mg/ kg of the Juglans regia septum of methanol extract (JRSME), and were monitored for 14 days. In subchronic study, JRSME was administered by gavage at dose of 1000 mg/kg daily in Wistar rats for 28 days. Antioxidant status and biochemical examinations were fulfilled, and the vital organs were subjected to pathological analyses. Results: The extract did not produce any toxic signs or deaths; the medium lethal dose must be higher than 5000 mg/kg. In subchronic study, No significant morphological and histopathological changes were observed in the studied tissues. There was a significant increase in serum malondialdehyde (MDA) level in treated group compared to control after 4 weeks of JRSME intake. The treatment of rats resulted in a significant reduction of serum urea level (p<0.05), kidney’s xanthine dehydrogenase (XDH) activity (p<0.001) and elevation of aldehyde oxidase (AO) activity (p<0.05) in kidney. In the treated group, the mean diameter of glomerulus and proximal urine tube epithelium stature was slightly greater than control group. A significant increase in serum MDA level is subject for further studies. Conclusion: This study showed that the extract has no acute or subacute adverse effects with dose of 1000 mg/kg. The administration of JRSME may improve kidney structure and function and help in treatment of some chronic diseases.
Item Type: | Article |
---|---|
Subjects: | Science Repository > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 19 Apr 2023 04:33 |
Last Modified: | 14 Sep 2023 07:57 |
URI: | http://research.manuscritpub.com/id/eprint/1996 |