Effect of BAP and IBA on in vitro Regeneration of Local Banana Variety of Sabri

The experiment was carried out to study the effect of benzylaminopurine (BAP) (1.0, 2.0, 3.0, 4.0 and 5.0 mg/L) and indole-3-butyric acid (IBA) (0.5, 1.0, 1.5, 2.0 and 2.5 mg/L) for in vitro regeneration of sabri variety of Banana using shoot tip explants. Highest response of explants (84%) and maximum number of shoots per explant (3.4) were observed with 5.0 mg/L BAP in sabri. In contrast, due to combined effect, 5.0 mg/L BAP+2.5 mg/L IBA showed best response (90%). The highest shoot number per explant (3.4) was found with 5.0 mg/L BAP+2.0 mg/L IBA. The maximum number of roots (3.4 and 5.2) was observed in 1.5 mg/L and 3.0 mg/L BAP+1.0 mg/L of IBA. In controlled environment, the regenerated healthy rooted plantlets were transferred from culture media to soil in plastic pots where 90% plantlets were survived and in open atmosphere, the survival rate was 88.89%. This protocol has the applicability in vitro rapid propagation of Banana.