Microsatellite DNA Markers for the Indian Rat Snake (Ptyas mucosa)

This chapter discusses the development of Microsatellite DNA markers for the Indian Rat Snake (Ptyas mucosa). One of the endangered snake species, the Indian Rat snake (Ptyas mucosa), for which thirteen microsatellite markers were generated in order to identify it, lives in Indian ecosystems that are currently the focus of extensive efforts to restore them. When 25 different people were evaluated, every locus exhibited polymorphism. The range of possible alleles at each locus was from 4 to 7, with 5.2 being the mean value. The usefulness of these markers for the research of the population genetic structure and diversity of this species was demonstrated by the fact that the observed and anticipated heterozygosity ranged from 0.76 to 1.00 and 0.59–0.94, respectively, and that the average polymorphic information content was found to be 0.664.