Growth Inhibition of Cancer Cell Line Using Fungal Secondary Metabolite

The purpose of this study was to compare the cytotoxic activity of the A. fumigaus virulence factors. Particularly in clinical isolates, Aspergillus fumigatus generates a number of toxic compounds, proteinaceous hemolysin, protease, and pigments like melanin that seem to act synergistically and additively on cells. In this work, gliotoxin, hemolysin, protease, and melanin were utilized in an experimental model to evaluate their cytotoxic activity by analyzing their effect on the Hep G2 cell line. Exposure times of 24 hours at three different doses of each component were employed, with triplicates of each concentration. A toxic effect was evident with a significant difference at the level of probability (p 0.05) for each purified compound, and this effect was contrasted across different concentrations for each purified compound. Growth inhibition of the Hep G2 cell line was gradually increased with the increase of compound concentration.