MAGNETIC FLUX AND GAMMA RAY CONJUNCTION IMPACTS WITH Beauvaria bassiana (BALSAMO) ON CERTAIN ASSESSMENTS OF Earias insulana (BOISD.)

Under laboratory conditions; the spiny bollworm, Earias insulana (Boisd.) treated with LC50,s of the fungal compound, Beauvaria bassiana (Balsamo) exposed to gamma doses (400 & 700 Gy) and prepared in magnetized water (180 mlt). Assays of toxicity, biological and biochemical of E. insulana treated as newly hatched larvae with B. bassiana in different six treatments were done. B. bassiana + 400 or 700 Gy + magnetized water were the most effective treatments on the most E. insulana larvae assays used. While, B. bassiana + 400 or 700 Gy without preparing in magnetized water had the lower effect comparing with the two aforementioned treatments. Meanwhile, B. bassiana treatment when used singly caused the least effective on E. insulana larvae different assays.

At 5- day post treatment, the compound of B. bassiana + 700 Gy + magnetized water was the best treatment caused the highly toxicity (LC50: 8x102 IU) on E. insulana larvae. Also, the same treatment had drastically biological effect on larval and pupal stages. Addition, total carbohydrate, followed by total lipid and proteins of E. insulana had drastically decreased with the mentioned treatment; while, free amino acids increased than untreated. Meanwhile, all the assayed enzymes had the highly decreased compared with untreated, especially aspartate amino transferase (AST/GOT), followed by alanine aminotransferase (ALT/GPT), acetyle cholinesterase, trehalase, invertase and amylase.

So, B. bassiana exposed to gamma doses (400 & 700 Gy) and prepared in magnetized water can potentiate the fungal compound used on E. insulana larvae to be effective than B. bassiana when used singly.