EARLY DETECTION OF SIGNIFICANT DISEASES IN EGGS AND LARVA OF SILKWORM WITH A MOLECULAR APPROACH: A CASE STUDY OF SOUTH SULAWESI

Silkworm (Bombyx mori L.) is an insect in Ordo Lepidoptera which produces cocoons before becoming an adult insect. Silkworm disease that causes decreased in cocoon production is Bombyx mori Nucleapolyhidrosisvirus (BmNPV) and pebrine. The Borcelina virus causes NPV disease, while pebrine caused by Nosema bombycis spores. This study aimed to identify NPV and N. bombycis in silkworms using a molecular approach. The materials used were 13 egg samples from strain C301 (Perum Perhutani, Soppeng District), strain PS01 (Puslitbang LHK, Bogor), strains S01, S02, S03, broodstock eggs, F1 hybrid (BPSKL Bili-Bili, Gowa District), and 14 larvae samples (strains C301, PS01, S01, S02, S03). The amplification results showed that during the egg phase of all samples were not detected with NPV while egg samples from strains S02 and S03 were detected with pebrine disease. While in the larval phase, NPV was detected in larvae PS01 4th instar (from Wajo District), 5th instar (from Soppeng District), and pebrine disease was detected in all larvae samples 3rd, 4th and 5th instars.