ANTIMICROBIAL ACTIVITY AND ANTI-BIOFILM FORMATION ON Pseudomonas aeruginosa ISOLATED FROM TONSILLITIS

This study aimed to investigate the prevalence of some antibiotic resistance among Pseudomonas aeruginosa by phenotypic and genotypic detection of some antibiotic resistance gene by PCR techniques. A total of 72 clinical samples were gathered of tonsillitis from AL-Hakeem Hospital and AL-Sadder Medical City in AL-Najaf province from March 2019 to May 2019. The findings of this research showed that 20/55 (36.36%) of isolates were identified as Pseudomonas aeruginosa, according to the cultural, microscopic, biochemical test and automated compact vitek-2 system using GN-ID card. Twenty of Pseudomonas aeruginosa isolates were tested for antimicrobial sensitivity. The findings of the current study showed that the antimicrobial strength levels of the 20 isolates Norffloxaxin (96.1%), Ciprofloxacin (96.1%), Tobromycin (92.3%) and Nalidixic acid (76.9%). The mild strength rate was found for Gentemycin (30.7%) and Cefotaxim (30.7%). Tetracyclin and amoxicillin (3.8% and 11.5%) respectively, was the smallest resistance rate.The findings of the present research showed that 10/14 (71.4 percent) were positive for the pslG gene out of the 14 isolates that yield positive outcomes in Congo red agar for biofilm formation.