DETECTION OF EBV, BRCA1 AND KI67 IN TRIPLE NEGATIVE BREAST CANCER (TNBC) INVASIVE DUCTAL CARCINOMA

The aim of this study was to detect EBV, BRCA1 and ki67 in triple negative breast cancer (TNBC) using Immunohistochemistry. One hundred formalin fixed paraffin embedded tissue blocks were collected from female patients previously diagnosed with breast cancer, seventy samples were triple negative (case) and thirty samples were non triple negative (control).The age of patients were ranged between 20 to 80 year with mean age 48.6 years. Histopathological diagnosis reveals that invasive ductal carcinoma was the most common among cases representing 84/100 (84%) and most of them were grade three 60/84 (75%).

Ki67 was positive in all samples 100% of cases while in controls were 16% (5/30). EBV was identified in 24.3% (17/70) of cases and 13.3 (4/30) of controls. BRCA1 gene was expressed in 22.9% (16/70) in case group while no expression among control group (0/30). TNBC had high proliferation index, there is association between BRCA1 gene expression and breast cancer status but less association with EBV infection.