Evaluation of the lethal dose of the methanol extract of rhizophora racemosa leaf using Karbers method

Aim: To evaluate the median lethal dose (LD50) of the methanol extract of Rhizophora racemosa leaf commonly used for toothache and dysmenorrhoea in the Niger Delta Area of Nigeria.

Methods: The modified arithmetic Karber’s method by Aliu and Nwude was used. Various doses of the extract were administered to adult albino rats through intraperitoneal route and the LD50 was calculated.

Results: The result revealed that the LD50 of the methanol extract was 1583.33 mg/kg which is within safe level of consumption.

Conclusion: This research therefore provides a scientific evidence that the traditional medicinal use of this plant is safe within the dose limit established.