Structural Change in Dimyristoyl Phosphatidyl Choline (DMPC) Molecule Alkyl Chains on the Addition of Cholesterol to DMPC Monolayer: Specific Addition Effect of Cholesterol

Ito, Daiki and Ikeda, Takanari and Taga, Keijiro and Yamamoto, Yasushi and Shervani, Zameer and Yamamoto, Masato (2019) Structural Change in Dimyristoyl Phosphatidyl Choline (DMPC) Molecule Alkyl Chains on the Addition of Cholesterol to DMPC Monolayer: Specific Addition Effect of Cholesterol. Journal of Biophysical Chemistry, 10 (02). pp. 15-29. ISSN 2153-036X

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Abstract

We have investigated the morphology of dimyristoyl phosphatidyl choline (DMPC)—cholesterol mixed monolayer formed on the water surface by dropping method using surface tension measurement (STm), Brewster angle microscopy (BAM), and infrared external reflection spectroscopy (IERS). STm results showed negative deviation of the limiting molecular area (A0) of cholesterol occurred when cholesterol was added to the DMPC monolayer. BAM images showed the expandable DMPC monolayer changed to the condensed rigid monolayer at more than cholesterol mole fraction (xChol) 0.4. IERS recordings showed that the addition of cholesterol at xChol = 0.4 occurred structural change from gauche- to trans- conformation of two DMPC molecule alkyl chains. From these results, it is found that cholesterol molecule has specific properties that cause structural transition of DMPC molecule alkyl chains.

Item Type: Article
Subjects: Science Repository > Chemical Science
Depositing User: Managing Editor
Date Deposited: 09 Mar 2023 06:53
Last Modified: 20 Sep 2023 06:31
URI: http://research.manuscritpub.com/id/eprint/900

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