Genome replication in asynchronously growing microbial populations

Pflug, Florian G. and Bhat, Deepak and Pigolotti, Simone and Igoshin, Oleg A (2024) Genome replication in asynchronously growing microbial populations. PLOS Computational Biology, 20 (1). e1011753. ISSN 1553-7358

[thumbnail of journal.pcbi.1011753.pdf] Text
journal.pcbi.1011753.pdf - Published Version

Download (1MB)

Abstract

Biological cells replicate their genomes in a well-planned manner. The DNA replication program of an organism determines the timing at which different genomic regions are replicated, with fundamental consequences for cell homeostasis and genome stability. In a growing cell culture, genomic regions that are replicated early should be more abundant than regions that are replicated late. This abundance pattern can be experimentally measured using deep sequencing. However, a general quantitative theory linking this pattern to the replication program is still lacking. In this paper, we predict the abundance of DNA fragments in asynchronously growing cultures from any given stochastic model of the DNA replication program. As key examples, we present stochastic models of the DNA replication programs in budding yeast and Escherichia coli. In both cases, our model results are in excellent agreement with experimental data and permit to infer key information about the replication program. In particular, our method is able to infer the locations of known replication origins in budding yeast with high accuracy. These examples demonstrate that our method can provide insight into a broad range of organisms, from bacteria to eukaryotes.

Item Type: Article
Subjects: Science Repository > Biological Science
Depositing User: Managing Editor
Date Deposited: 23 Mar 2024 08:46
Last Modified: 23 Mar 2024 08:46
URI: http://research.manuscritpub.com/id/eprint/4026

Actions (login required)

View Item
View Item