Degradative Capability of Microbial Culture, Carrot Peel Waste and Carbon Dot in Remediation of Petroleum Hydrocarbon in Soil

Aim: To determine the degradative capability of microbial culture, carrot peel waste and carbon dot in remediation of petroleum hydrocarbon in soil.

Study Design: Microbial culture (MC), organic (Carbon dot and Carrot peel) amendments were used in stimulating and remediating the impacted soil.

Place and Duration of Studies: Environmental Management and Toxicology, Federal University of Petroleum Resource, Effurun. Nigeria. One Month.

Methodology: Physicochemical parameters were done on theentire samples. Totalcultural heterotrophic bacteria (TCHB) and hydrocarbon utilizing bacteria (HUB).Isolates were identified using their macroscopic, microscopic and biochemical characteristics. A total of ten bacteria species where identified, three were used as consortium which include,Arthrobacter specie, Pseudomonas species and, Bacillus species. Characterization of the Coconut husk, revealed its authenticity, elemental composition and the peak, it exhibited photoluminescence under the ultra violet fluorescent light. Microbial culture (MC), organic (Carbon dot and Carrot peel) amendments were used in stimulating theimpacted soil.

Results: The total petroleum hydrocarbon for the polluted and control samples was 2018 mg/kg and 0.003 mg/kg respectively. The concentration in polluted sample are 11.62, and 8.50 ( mg/kg) while for control sample are <0.001, and <0.001 ( mg/kg) ..It was observed that within the first and the last week of exposure the values of TPH reduced drastically when used singly and in combination, with microbial culture, Carrot peel and Carbon dot having the insignificant values of (0.028±0.002), CMC (0.044±0.003) and Carrot peel 0.007±0.002).for the combined amendment,TPH was so drastic that it was almost within detectable limitwith concentration C (0.005±0.002),B (0.017±0.002) and A (0.055±0.002) Statistical analysis revealed there was significant difference (at P =05) in organic carbon values with respect to the different amendments.

Conclusion: Studies have showed that using biostimulation and bioaugumentation is a good practice for cleaning up soiled environment, this practice is safe, eco-friendly and cost effective.